The St. Johns culture was an archaeological culture in northeastern Florida, USA that lasted from about 500 BCE (the end of the Archaic period) until shortly after European contact in the 17th century. The St. Johns culture was present along the St. Johns River and its tributaries (including the Oklawaha River, and along the Atlantic coast of Florida from the mouth of the St. Johns River south to a point east of the head of the St. Johns River, near present-day Cocoa Beach, Florida. At the time of first European contact, the St. Johns culture area was inhabited by speakers of the Mocama (or Agua Salada), Agua Fresca and Acuera dialects of the Timucua language and by the Mayacas.

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